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宿主来源
Rabbit抗原名称
FMC63 scFv免疫原
Recombinant Protein克隆号
S-2163-77抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM稀释度
应用 稀释度 推荐种属 FCM 1:2000 Species independent
FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.
流式分析
Flow cytometric analysis of CAR (FMC63 scFv) transfected 293T (Human embryonic kidney epithelial cell (Right panel) or untrasfected 293T (Left panel) labelling FMC63 antibody at 1/2000 (0.1 μg) dilution. Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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