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宿主来源
Mouse抗原名称
CD14分子别名
Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein免疫原
Recombinant Protein细胞定位
Secreted, Cell membraneAccession
P08571克隆号
S-631-50抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1.25μl per million cells in 100μl volume | Hu |
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.
流式分析
Flow cytometric analysis of Human CD14 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with APC Mouse IgG1, κ Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD14 Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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