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宿主来源
Rat抗原名称
PD-1 (CD279)分子别名
Programmed cell death protein 1; Protein PD-1; mPD-1; CD279; Pd1; Pdcd1细胞定位
Cell membraneAccession
Q02242克隆号
S-R496抗体类型
Rat mAb抗体同种型
IgG2a同型对照
S0B0932反应种属 ?
Ms阳性样本
EL4.IL-2纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms FCM 1:2000 Ms
Programmed cell death protein 1 (PD-1), also known as CD279, is a type I transmembrane protein and a member of the CD28 immunoglobulin superfamily, encoded by the _PDCD1_ gene. It is primarily expressed on the surface of various immune cells, including T cells, B cells, macrophages, dendritic cells, and natural killer cells. PD-1 plays a crucial role in regulating immune responses by binding to its ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC), which are expressed on antigen-presenting cells and tumor cells. When PD-1 interacts with its ligands, it triggers inhibitory signals that downregulate T cell activity, preventing excessive immune responses and promoting self-tolerance. However, this pathway can also be exploited by cancer cells to evade immune surveillance, leading to tumor progression. As a result, PD-1 has become a key target in cancer immunotherapy, with several anti-PD-1 antibodies approved for treating various cancers, achieving significant and durable efficacy in some patients.
免疫印迹
WB result of Rat Anti-Mouse PD-1 (CD279) Antibody
Primary antibody: Rat Anti-Mouse PD-1 (CD279) Antibody at 1/1000 dilution
Lane 1: recombinant PD-1 Fc Chimera 10 ng
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 55-70 kDaWB result of Rat Anti-Mouse PD-1 (CD279) Antibody
Primary antibody: Rat Anti-Mouse PD-1 (CD279) Antibody at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: EL4.IL-2 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 40-55 kDa
流式分析
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast, Left) / EL4.IL-2 (Mouse lymphoma T lymphocyte, Right) labelled with anti-Mouse PD-1 (CD279) antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat IgG2a (Black) Isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: NIH/3T3
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