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Rabbit抗原名称
CD25分子别名
Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra免疫原
Recombinant Protein细胞定位
MembraneAccession
P01590克隆号
SDT-2234-65抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
CTLL-2纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IHC-P 1:100-1:200 Ms ICC 1:500 Ms
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
免疫印迹
WB result of CD25/IL-2Rα Recombinant Rabbit mAb
Primary antibody: CD25/IL-2Rα Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: CTLL-2 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate; C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 38-70 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD25/IL-2Rα antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-CD25/IL-2Rα antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in CTLL-2 cells (top panel) and negative staining in C2C12 cells (below panel). Anti-CD25/IL-2Rα antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CD25/IL-2Rα in mouse tissue.
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