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FGFR2b Recombinant Rabbit mAb (SDT-423-121)

FGFR2IIIb,Fibroblast growth factor receptor 2b,Fibroblast growth factor receptor 2IIIb

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2359
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    FGFR2b

  • 分子别名

    FGFR2IIIb; Fibroblast growth factor receptor 2b; Fibroblast growth factor receptor 2IIIb

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted, Cell membrane

  • Accession

    P21802-3

  • 克隆号

    SDT-423-121

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    KATO III

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IHC-P 1:1000 Hu
背景介绍

  • FGFR2b is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR2b has important roles in embryonic development and tissue repair, especially bone and blood vessels. Recent studies have found that about 30% of patients overexpress FGFR2b protein, which may be used as a new biomarker and research target for gastric cancer.

  • 免疫印迹

    • WB result of FGFR2b Recombinant Rabbit mAb
      Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: SW620 whole cell lysate 20 µg
      Lane 2: KATO III whole cell lysate 20 µg
      Negative control: SW620 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
      Observed MW: 90~145 kDa

    • WB result of FGFR2b Recombinant Rabbit mAb
      Primary antibody: FGFR2b Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: recombinant human FGFR2b protein 10 ng (CAT. NO. UA010145)
      Lane 2: recombinant human FGFR2c protein 10 ng (UniProt: P21802-1)
      Lane 3: recombinant human FGFR1 protein 10 ng (CAT. NO. UA020008)
      Lane 4: recombinant human FGFR3 protein 10 ng
      Lane 5: recombinant human FGFR4 protein 10 ng (CAT. NO. UA020009)
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa
      Observed MW: 38~60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human gastric cancer (case 1). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows negative staining in paraffin-embedded human gastric cancer (case 2). Anti- FGFR2b antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of FGFR2b in tumor tissue.

    • Expression of FGFR2b in human tissue.

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