PMEL/Melanoma gp100 Recombinant Rabbit mAb (SDT-434-70)

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货号： S0B2217

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

PMEL/Melanoma gp100
分子别名

ME20-M, ME20M, Pmel 17, P1, P100, Premelanosome protein, Silver locus protein homolog, Melanosome, HMB45
免疫原

Synthetic Peptide
细胞定位

Melanosome
Accession

P40967
克隆号

SDT-434-70
抗体类型

Recombinant mAb
应用

IHC-P, ICC, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
ICC	1:250
IHC-P	1:1000

背景介绍

PMEL encodes a key component of the melanosome, the organelle essential for melanin synthesis, storage and transport [PMID: 30561643IF: 3.1 Q2 B2]. Melanoma gp100 is a protein highly expressed in melanoma tissue.

免疫印迹
- WB result of PMEL/Melanoma gp100 Rabbit mAb Primary antibody: PMEL/Melanoma gp100 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: SK-MEL-28 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 70 kDa Observed MW: 100,26 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human melanoma. Anti-PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human transitional cell carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human prostatic carcinoma. Anti- PMEL/Melanoma gp100 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in SK-MEL-28 cells. Anti-PMEL/Melanoma gp100 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control:ICC shows negative staining in HeLa cells. Anti-PMEL/Melanoma gp100 antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).