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Rabbit抗原名称
MLH1分子别名
DNA mismatch repair protein Mlh1, MutL protein homolog 1, COCA2免疫原
Synthetic Peptide细胞定位
NucleusAccession
P40692克隆号
SDT-112-50-2抗体类型
Recombinant mAb应用
ICC, WB反应种属 ?
Hu预测反应种属
(反应种属缩写表)Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| ICC | 1:50 |
DNA mismatch repair protein Mlh1 or MutL protein homolog 1 is a protein that in humans is encoded by the MLH1 gene located on chromosome 3. It is a gene commonly associated with hereditary nonpolyposis colorectal cancer. MLH1 protein is one component of a system of seven DNA mismatch repair proteins that work coordinately in sequential steps to initiate repair of DNA mismatches in humans [PMID: 18543306]. Defects in mismatch repair, found in about 13% of colorectal cancers, are much more frequently due to deficiency of MLH1 than deficiencies of other DNA mismatch repair proteins [PMID: 15887099]. The seven DNA mismatch repair proteins in humans are MLH1, MLH3, MSH2, MSH3, MSH6, PMS1 and PMS2 [ PMID: 18543306].
免疫印迹
WB result of MLH1 Rabbit mAb
Primary antibody: MLH1 Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 84 kDa
(This blot was developed with high sensitivity substrate)
免疫组化
WB result of MLH1 Rabbit mAb Primary antibody: MLH1 Rabbit mAb at 1/1000 dilution Lane 1: HCT 116 whole cell lysate 20 µg Lane 2: HeLa whole cell lysate 20 µg Lane 3: Jurkat whole cell lysate 20 µg Negative control: HCT 116 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 84 kDa Observed MW: 84 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-MLH1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in HCT-116 cells. Anti-MLH1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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