Claudin18.2 + Claudin18.1 Recombinant Rabbit mAb (SDT-298-29)

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货号： S0B2191

Datasheet COA

价格： ￥700
规格：
25ul100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

CLAUDIN18.2 + CLAUDIN18.1
分子别名

N/A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P56856
克隆号

SDT-298-29
抗体类型

Recombinant mAb
应用

IHC-P, ICC
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标签

N/A
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:1000
ICC	1:500

背景介绍

Claudin 18 is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. The claudin-18 gene has two splice variants: claudin 18.1, which is found primarily in the lung, and claudin 18.2, which is expressed exclusively in the stomach.

免疫组化
- IHC shows positive staining in paraffin-embedded human lung. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in KATOIII cells. Anti-Claudin18.2 + Claudin18.1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).