S-RMab^® Bcl-2 Recombinant Rabbit mAb (SDT-R160)

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货号： S0B2181

Datasheet COA

价格： ￥600
规格：
25μl100 μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

Bcl-2
分子别名

B-cell lymphoma-2, Apoptosis regulator Bcl-2
免疫原

N/A
细胞定位

Nucleus membrane, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Cytoplasm
Accession

P10415
克隆号

SDT-R160
抗体类型

Recombinant mAb
应用

IHC-P, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:1000

背景介绍

Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis. It was the first apoptosis regulator identified in any organism. Bcl-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.

免疫印迹
- WB result of Bcl-2 Rabbit mAb Primary antibody: Bcl-2 Rabbit mAb at 1/1000 dilution Lane 1: Daudi whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Lane 3: U-937 whole cell lysate 20 µg Lane 4: HL-60 whole cell lysate 20 µg Lane 5: THP-1 whole cell lysate 20 µg Negative control: Daudi whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26kDa Observed MW: 26kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human mantle cell lymphoma. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Bcl-2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.