S-RMab^® alpha smooth muscle Actin (SMA) Recombinant Rabbit mAb (SDT-301-142)

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货号： S0B2163

Datasheet COA

价格： ￥600
规格：
25μl100 μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

alpha smooth muscle Actin (SMA)
分子别名

Alpha-actin-2, ACTA2, Smooth muscle actin, actin-sm, ASM-1, CGA7, ASM/H12
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

P62736
克隆号

SDT-301-142
应用

IHC-P, WB, IF
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:1000
IF	1:25

背景介绍

Alpha smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in non-muscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.

免疫印迹
- WB result of SMA Rabbit mAb Primary antibody: SMA Rabbit mAb at 1/1000 dilution Lane 1: mouse lung lysate 20 µg Lane 2: mouse stomach lysate 20 µg Negative control: mouse lung lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa Observed MW: 42 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/25 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue).Counterstain with tubulin (red). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/25 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue).Counterstain with tubulin (red). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/25 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue).Counterstain with tubulin (red). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.