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Rabbit抗原名称
CK5/6分子别名
Keratin 5/6, Cytokeratin 5/6免疫原
N/A细胞定位
Cytoplasm克隆号
SDT-P004抗体类型
Rabbit mAb应用
IHC-P, WB, IF反应种属 ?
Hu纯化方式
Protein A浓度
0.2 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:1000 |
| WB | 1:5000 |
| IF | 1:500 |
The molecular weights of CK5/6 (Cytokeratin 5/6) are 56kDa and 58kDa. Expressed in basal and stratum spinosum of the skin, as well as in the prostate basal cells and squamous epithelial cells. It can be used for the differential diagnosis of squamous cell carcinoma and adenocarcinoma, as well as mesothelioma and adenocarcinoma. It combined with p63 and AMACR/p504s for diagnosis of benign and malignant prostatic lesions.
免疫印迹
WB result of CK5/6 Rabbit mAb
Primary antibody: CK5/6 Rabbit mAb at 1/5000 dilution
Lane 1: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56, 58 kDa
Observed MW: 56, 58 kDa
Exposure time: 5s
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
IHC shows positive staining in paraffin-embedded human breast invasive ductal carcinoma. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK5/6 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
(This IHC was developed using Immuno-Histo Stainer: PA-3600.)
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostate. Anti-CK5/6 antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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