大包装询价 产品介绍 引用文献(0) 评论(0)
宿主来源
Rabbit抗原名称
WT1分子别名
Wilms tumor protein; WT33; WT1细胞定位
Nucleus, CytoplasmAccession
P19544克隆号
SDT-2864抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
The Wilms Tumor Protein (WT1) is a transcription factor that plays a crucial role in the development and differentiation of various tissues, particularly in the kidneys and gonads. It is encoded by the WT1 gene and is essential for normal nephrogenesis, ensuring the proper formation of the glomeruli and other renal structures. Abnormal expression or mutations in the WT1 gene are associated with Wilms tumor, a pediatric kidney cancer, as well as other developmental disorders such as Denys-Drash syndrome and Frasier syndrome. In addition to its role in development, WT1 is also involved in the regulation of cell proliferation, apoptosis, and the maintenance of mesenchymal stem cells. Its expression is tightly regulated and has been studied extensively as a potential biomarker and therapeutic target in cancer research.
免疫印迹
WB result of Wilms Tumor Protein (WT1) Recombinant Rabbit mAb
Primary antibody: Wilms Tumor Protein (WT1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: DU 145 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: OVCAR-3 whole cell lysate 20 µg
Negative control: DU 145 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 55 kDaWB result of Wilms Tumor Protein (WT1) Recombinant Rabbit mAb
Primary antibody: Wilms Tumor Protein (WT1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 53 kDaWB result of Wilms Tumor Protein (WT1) Recombinant Rabbit mAb
Primary antibody: Wilms Tumor Protein (WT1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 53 kDa
流式分析
![item.picture[0].fileOrgName](https://univ-sdt.oss-cn-shanghai.aliyuncs.com/prod/202304/daef8a77133e4fa38bb9740a8fcd524c.jpg)
Flow cytometric analysis of K-562 cells labelling WT1 antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded Wilms tumor. Anti-Wilms Tumor Protein (WT1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Wilms Tumor Protein (WT1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Wilms Tumor Protein (WT1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Wilms Tumor Protein (WT1) in tumor tissue.
Expression of Wilms Tumor Protein (WT1) in human tissue.
引用文献(0)
评论(0)