S-RMab^® Mesothelin Recombinant Rabbit mAb (SDT-R070)

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货号： S0B2104

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Mesothelin
分子别名

CAK1 antigen, MSLN
免疫原

N/A
细胞定位

Cell membrane, Cytoplasm
Accession

Q13421
克隆号

SDT-R070
抗体类型

Rabbit mAb
应用

IHC-P, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:1000

背景介绍

Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein normally expressed in mesothelial cells that line the pleura, peritoneum, and pericardium. The MSLN gene encodes a precursor protein of 71 kDa that is processed to a 31 kDa shed protein called MPF (megakaryocyte potentiating factor) and a 40 kDa membrane bound protein, mesothelin.Mesothelin is reported to be highly expressed in several types of malignant tumors, such as malignant mesothelioma, ovarian cancer, pancreatic adenocarcinoma, and lung adenocarcinoma.

免疫印迹
- WB result of Mesothelin Rabbit mAb
  Primary antibody: Mesothelin Rabbit mAb at 1/500 dilution
  Lane 1: A549 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Negative control: A549 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 40, 69 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human mesothelioma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.