S-RMab^® Keratin 10 Recombinant Rabbit mAb (SDT-R067)

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货号： S0B2098

Datasheet COA

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产品规格

宿主来源

Rabbit
抗原名称

Keratin 10
分子别名

Cytokeratin-10, CK-10, KRT10
免疫原

N/A
Accession

P13645
克隆号

SDT-R067
抗体类型

Rabbit mAb
应用

IHC-P, WB, IP
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.125 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000-1:2000
IHC-P	1:1000
IP	1:25

背景介绍

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Keratin 10 plays a role in the establishment of the epidermal barrier on plantar skin.

免疫印迹
- WB result of Keratin 10 Rabbit mAb
  Primary antibody: Keratin 10 Rabbit mAb at 1/1250 dilution
  Lane 1: Raw 264.7 whole cell lysate 20 µg
  Lane 2: mouse skin lysate 20 µg
  Negative control: RAW 264.7 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
  Exposure time: 7s
免疫沉淀
- Keratin 10 Rabbit mAb at 1/25 dilution (0.5 µg) immunoprecipitating Keratin 10 in 0.2 mg K562 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Keratin 10 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: K562 whole cell lysate 5 µg (Input)
  Lane 2: Keratin 10 Rabbit mAb IP in K562 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
  Exposure time: 90 s
免疫组化
- IHC shows positive staining in paraffin-embedded human skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.