S-RMab^® CD45 Recombinant Rabbit mAb (SDT-R035)

Product Introduction

• Host

  Rabbit

• Antigen

  CD45

• Synonyms

  Leukocyte common antigen (L-CA)

• Immunogen

  N/A

• Location

  Cell membrane

• Accession

  P08575

• Clone Number

  SDT-R035

• Antibody Type

  Rabbit mAb

• Application

  IHC-P, ICC, WB

• Reactivity

  Hu

• Purification

  N/A

• Concentration

  0.25 mg/ml

• Conjugation

  Unconjugated

• Physical Appearance

  Liquid

• Storage Buffer

  PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

• Stability & Storage

  12 months from date of receipt / reconstitution, -20 °C as supplied

• • WB

    1:2500

  • IHC-P

    1:250-1:1000

  • ICC

    1:250

• CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.

• Western Blot

  • 

    WB result of CD45 Rabbit mAb                    

    Primary antibody: CD45 Rabbit mAb at 1/2500 dilution
    Lane 1: MCF7 whole cell lysate 20 µg
    Lane 2: Raji whole cell lysate 20 µg
    Lane 3: Jurkat whole cell lysate 20 µg
    Negative control: MCF7 whole cell lysate
    Low expression control: Raji whole cell lysate    

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 180~240 kDa
    Observed MW: 240~260 kDa
    Exposure time: 30s 

• Immunohistochemistry

  • 

    IHC shows positive staining in paraffin-embedded human tonsil.

    Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human spleen.

    Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human colon.

    Anti-CD45 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human colon.

    Anti-CD45 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human cervix cancer.

    Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protoco

  • 

    IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma.

    Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    Negative control:IHC shows negative staining in paraffin-embeddedhuman skeletal muscle.

    Anti-CD45 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.                      

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

• Immunocytochemistry

  • 

    ICC shows positive staining in Jurkat cells. Anti-CD45 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

  • 

    Negative control:ICC shows negative staining in MCF7 cells. Anti-CD45 antibody was used at 1/250 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).