S-RMab^® MUC6 Recombinant Rabbit mAb (SDT-081-72)

浏览次数：27

货号： S0B2081

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

申请试用 大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

MUC6
分子别名

MUC-6, Gastric mucin-6
免疫原

Synthetic Peptide
细胞定位

Secreted
Accession

Q6W4X9
克隆号

SDT-081-72
抗体类型

Rabbit mAb
应用

IHC-P, IF
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:400
IF	1:400

背景介绍

Mucin 6, oligomeric mucus/gel-forming, also known as MUC6. The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Normal gastric mucosa also expresses MUC1, MUC4, MUC5AC, and MUC6, with MUC1, MUC5AC, and MUC6 being the most prominent. MUC6 is expressed in mucopeptic cells of the neck region of the body and in pyloric glands of the antrum. It may provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. MUC6 also play an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers and participates in epithelial organogenesis.

免疫组化
- IHC shows positive staining in paraffin-embedded human stomach.
  Anti-MUC6 antibody was used at 1/400 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negaitive staining in paraffin-embedded human colon.
  Anti-MUC6 antibody was used at 1/400 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human stomach. Anti-MUC6 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- Negative control: IF shows negative staining in paraffin-embedded human colon. Anti-MUC6 antibody was used at 1/400 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.