S-RMab^® PD-L1 Recombinant Rabbit mAb (SDT-119-38)

Product Introduction

• Host

  Rabbit

• Antigen

  PD-L1

• Synonyms

  CD274, B7-H1

• Immunogen

  Recombinant Protein

• Location

  Membrane

• Accession

  Q9NZQ7

• Clone Number

  SDT-119-38

• Antibody Type

  Rabbit mAb

• Application

  IHC-P

• Reactivity

  Hu

• Purification

  Protein A

• Concentration

  0.5 mg/ml

• Physical Appearance

  Liquid

• Storage Buffer

  PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

• Stability & Storage

  12 months from date of receipt / reconstitution, -20 °C as supplied

• • IHC-P

    1:100

• Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM). This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) - further mediated by a lower regulation of the gene Bcl-2. Upregulation of PD-L1 on immune cells (especially myeloid cells) can also lead to formation of an immunosuppressive environment in a highly localized manner that also allow the cancer cells to proliferate.

• Immunohistochemistry

  • 

    IHC shows positive staining in paraffin-embedded human tonsil.

    Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human placenta.

    Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    eat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human lung cancer.

    Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human lung cancer.

    Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    eat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human cervix cancer.

    Anti-PD-L1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Counterstained with hematoxylin.

    Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.