SOX2 Recombinant Rabbit mAb,PBS Only (SDT-066-58)

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货号： S0B2051P

Datasheet COA

价格：询价
规格：
1mg
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

SOX2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P48431
克隆号

SDT-066-58
抗体类型

Rabbit mAb
应用

ICFCM, IHC-P, ICC, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

1 mg/ml
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt, 4°C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:2000
ICC	1:500
ICFCM	1:500

背景介绍

SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential for maintaining self-renewal, or pluripotency, of undifferentiated embryonic stem cells. Sox2 has a critical role in maintenance of embryonic and neural stem cells. Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids.

免疫印迹
- WB result of sox2 Rabbit mAb
  Primary antibody: sox2 Rabbit mAb at 1/1000 dilution
  Lane 1: NCCIT lysate 20 µg
  Lane 2: MCF7 bladder lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 35 kDa
  
  Lane 2: 40s
流式分析
- Flow cytometric analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX2 antibody at 1/500 (0.1 μg) dilution / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti –Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach.
  Anti-SOX2 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human lung.
  Anti-SOX2 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer.
  Anti-SOX2 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human seminoma.
  Anti-SOX2 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in NCCIT cells. Anti-SOX2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).