S-RMab^® CD21 Recombinant Rabbit mAb(SDT-007-47)

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货号： S0B2043

Datasheet COA

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

CD21
分子别名

CR2,CD3R, Complement C3d receptor, Epstein-Barr virus receptor (EBV receptor)
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

P20023
克隆号

SDT-007-47
抗体类型

Rabbit mAb
抗体同种型

IgG
应用

IHC-P, FCM, ICC, WB
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.08mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:1000
FCM	1:80
ICC	1:50
WB	1:200

背景介绍

CD21 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.B cells express CD21 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. CD21 interacts with CD19 and on mature B cells forms a complex with CD81 (TAPA-1). The CD21-CD19-CD81 complex is often called the B cell co-receptor complex, because CD21 binds to opsonized antigens through attached C3d (or iC3b or C3dg) when the B-cell receptor binds antigen. This results in the B cell having greatly enhanced response to the antigen. Epstein-Barr virus (EBV) can bind CD21, enabling EBV to enter and infect B cells.

验证数据
- Flow cytometric analysis of Raji cells labelling CD21 antibody at 1/80 dilution (0.1ug)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody.
免疫印迹
- WB result of CD21 Rabbit mAb
  Primary antibody: CD21 Rabbit mAb at 1/200 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: Daudi whole cell lysate 20 µg
  Lane 3: Raji whole cell lysate 20 µg
  Negative control: K562 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 113 kDa
  Observed MW: 160 kDa
  Exposure time: 20s
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD21 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-CD21 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative tissue: IHC shows negative staining in paraffin-embedded human kidney. Anti-CD21 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD21 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD21 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Ramos cells. Anti-CD21 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).