Aurora-B Recombinant Rabbit mAb (SDT-079-32)

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货号： S0B2034

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

Aurora-B
分子别名

Aurora 1, AIM-1, ARK-2, STK-1, Aurora-related kinase 2
免疫原

Synthetic Peptide
Accession

Q96GD4
克隆号

SDT-079-32
抗体类型

Rabbit mAb
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IP	1:25
IHC-P	1:500-2000
WB	1:1000
ICC	1:500
ICFCM	1:5000

背景介绍

Aurora kinase B is a protein that functions in the attachment of the mitotic spindle to the centromere. The expression and activity of Aurora B are regulated according to the cell cycle. Expression of Aurora B reaches a maximum at the G2-M transition, whereas Aurora B protein is most active during mitosis. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes. In cancerous cells, over-expression of these enzymes causes unequal distribution of genetic information, creating aneuploid cells, a hallmark of cancer.

免疫印迹
- WB result of Aurora-B Rabbit mAb
  Primary antibody : Aurora-B Rabbit mAb at 1/1000 dilution
  Lane 1: Hela whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: Raji whole cell lysate 20 µg
  Lane 4: T47D whole cell lysate 20 µg
  Lane 5: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 39 kDa
  Exposure time: 16 s
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Aurora-B antibody at 1/5000 dilution (0.01 μg)/ (Right) compared with a Rabbit monoclonal IgG / (Left) isotype control. Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Cells were co-stained with DAPI to differentiate cell cycle phase.
免疫沉淀
- Aurora-B Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Aurora-B in 0.4mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using Aurora-B Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1 : HeLa whole cell lysate 10µg (input)
  Lane 2 (+): Aurora-B Rabbit mAb IP in HeLa whole cell lysate
  Lane 3 (-): Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 41 kDa
  Observed MW: 39 kDa
  Exposure time: 10s
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil.
  Anti-Aurora B antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervix cancer.
  Anti-Aurora B antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-Aurora-B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).