SCGN Recombinant Rabbit mAb,PBS Only (SDT-040-69)

浏览次数：7

货号： S0B2018P

Datasheet COA

价格：询价
规格：
1mg
数量：

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

SCGN
分子别名

Secretagogin, SECRE
免疫原

Synthetic Peptide
Accession

O76038
克隆号

SDT-040-69
抗体类型

Rabbit mAb
应用

IHC-P, IF
反应种属 ?

Hu, Ms
纯化方式

Protein A
研究领域

Neuroscience
浓度

1 mg/ml
分子量

32kDa
标记

Unconjugated
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt, 4°C as supplied

稀释度

应用	稀释度
IHC-P	1:2000
IF	1:1000

背景介绍

Secretin (SCGN) is a cytoplasmic calcium binding protein, which has homology with calcium binding protein-D28k (CALB1) and calcium reticulum protein (CALB2). Secretin protein was isolated from Langerhans Island and insulinoma cells, and was highly expressed in pancreas, but decreased in thyroid, adrenal medulla, stomach, small intestine and colon. The cloning and sequence analysis of secretin cDNA showed that the corresponding protein included six EF hand calcium binding domain tandem repeats. The interaction between secretin and vesicular transporters (including SNAP25, SNAP23, ARFGAP2 and DOC2A) suggests that SCGN may play a role in the release of neurotransmitters and hormones. The study on the expression of secretin in fetal brain and developing brain shows that this calcium binding protein plays a role in differentiation, brain wiring and neuronal development.

免疫组化
- IHC shows positive staining in paraffin-embedded human pancreas. Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat.
  Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon.
  Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human kidney(negative tissues).
  Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma(negative tissues).
  Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse pancreas.
  Anti-SCGN antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse pancreas. Anti-SCGN antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.