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抗原名称
GFP分子别名
Green fluorescent protein应用
Protein purification ,Co-IP ,IP交叉反应
Can also enrich GFP variants such as CFP, ECFP, YFP, BFP, aceGFP, EGFP, Clover, Citrine, EYFP and Venus.
性状
Liquid缓冲体系
50% slurry in PBS pH7.4, 0.05% Proclin 300. Ensure thorough mixing before each use. Wide-mouth pipette tips are recommended. In case of difficulty in aspiration due to improper operation, additional Storage Buffer can be added at your discretion.储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IP | 25ul/test |
Anti-GFP nanobody agarose beads is an affinity chromatography medium designed for easy, one-step purification of recombinant GFP fusion proteins in E. coli, insect cells and mammalian cells. The recombinant GFP fusion proteins can be purified directly from pre-treated cell lysate using Anti-GFP nanobody agarose beads. It is the excellent choice for high performance purifications.
电泳
Anti-GFP Nanobody Agarose Beads is used to immunoprecipitated GFP-fusion proteins from Escherichia coli lysate supernatant. During elution with Acidic elution buffer, the enriched GFP-fusion proteins are released from Anti-GFP Nanobody Agarose Beads.
SDS-PAGE analysis was performed.
Lane 1: Input
Lane 2: Flow-through
Lane 3: GFP-fusion protein eluted by acidic buffer
Lane 4: Agarose Beads after elution
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