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Rabbit抗原名称
α-Actinin-1分子别名
Alpha-actinin-1; ACTN1免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, CytoskeletonAccession
P12814克隆号
SDT-311-20抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, NIH/3T3, C6纯化方式
Protein A浓度
2 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
Alpha-actinin-1 is a member of the spectrin superfamily of proteins and is an actin-binding protein with multiple roles in different cell types1. It is a component of the cytoskeleton and is involved in cross-linking actin filaments, which helps in maintaining cell shape, providing mechanical strength, and playing a role in cell motility and signal transduction. In nonmuscle cells, alpha-actinin-1 is found along microfilament bundles and adherens-type junctions, where it binds actin to the membrane. It is also present in muscle cells, where it helps anchor the myofibrillar actin filaments at the Z-discs and dense bodies. This protein is highly conserved and has been implicated in various biological processes, including cell migration, cell division, and the maintenance of cell structure. It is expressed ubiquitously and has been associated with several diseases. For instance, alpha-actinin-1 has been linked to myopathies, neurodegenerative disorders, and kidney diseases. It may also play a role in cancer cell progression and metastasis, as well as in the migration of immune cells. In terms of autoimmune diseases, alpha-actinin-1 has been identified as a potential target autoantigen, particularly in systemic lupus erythematosus (SLE) and autoimmune hepatitis. It is suggested that antibodies against alpha-actinin-1 may contribute to the pathogenesis of these diseases by cross-reacting with other proteins.
免疫印迹
WB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: HeLa whole cell lysate 20 µg; HepG2 whole cell lysate 20 µg
Primary antibody:
Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 103 kDa
Observed MW: 103 kDaWB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: NIH/3T3 whole cell lysate 20 µg
Primary antibody:
Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 103 kDa
Observed MW: 103 kDaWB result of α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: C6 whole cell lysate 20 µg
Primary antibody:
Lane 1: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate)) at 1/5000 dilution
Lane 2: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: α-Actinin-1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 103 kDa
Observed MW: 103 kDa
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