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Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb (S-1874-69)

Serine/threonine-protein kinase LATS1,Large tumor suppressor homolog 1,WARTS protein kinase (h-warts),WARTS,Serine/threonine-protein kinase LATS2,Kinase phosphorylated during mitosis protein,Large tumor suppressor homolog 2,Serine/threonine-protein kinase kpm,Warts-like kinase,KPM

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1465
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-LATS1/2(Ser909/872)

  • 分子别名

    Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase (h-warts); WARTS; Serine/threonine-protein kinase LATS2; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm; Warts-like kinase; KPM

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Endoplasmic reticulum

  • Accession

    O95835, Q9NRM7

  • 克隆号

    S-1874-69

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    HeLa treated with 100 ng/ml Calyculin A for 30 minutes, serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms
    ICC 1:500 Hu
背景介绍

  • Phospho-LATS1/2(Ser909/872) refers to the phosphorylated forms of the mammalian Hippo signaling pathway kinases LATS1 (Large Tumor Suppressor 1) and LATS2 at specific serine residues (Ser909 in LATS1 and Ser872 in LATS2). As core components of this pathway, LATS1/2 are activated through phosphorylation by upstream kinases MST1/2, which subsequently phosphorylate downstream effector proteins YAP/TAZ to regulate their nucleocytoplasmic shuttling, degradation, and biological functions, thereby suppressing cell proliferation and promoting apoptosis. Phosphorylation at Ser909/872 is likely a critical step in LATS1/2 activation, potentially modulating their kinase activity, stability, or interactions with regulatory proteins. This phosphorylation event is commonly used as a biomarker of Hippo pathway activity, detectable via specific antibodies (e.g., in Western blotting), and is studied in contexts such as cancer and organ development due to the pathway's strong association with tumorigenesis and metastasis. Notably, species-specific variations or splice isoforms may alter residue numbering, and functional validation often involves mutagenesis experiments (e.g., phosphomimetic glutamic acid substitutions or non-phosphorylatable alanine mutations) to assess impacts on pathway regulation.

  • 免疫印迹

    • WB result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
      Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 127, 120 kDa
      Observed MW: 140, 120 kDa

    • WB result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
      Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: serum-starved NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 127, 120 kDa
      Observed MW: 130, 115 kDa

  • 斑点杂交

    • Dot blot result of Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb
      Lane 1: LATS1/2(Ser909/872) phospho peptide
      Lane 2: LATS1/2(Ser909/872) unmodified peptide
      Primary antibody: Phospho-LATS1/2(Ser909/872) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with 100 ng/ml Calyculin A for 30 minutes (top panel) and untreated HeLa cells (below panel). Anti- Phospho-LATS1/2(Ser909/872) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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