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Rabbit抗原名称
Phospho-RIP (Ser166)分子别名
Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); RIP; RIP1; RIPK1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
Q13546克隆号
S-1843-37抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu
Phospho-RIPK1 (Ser166) is a phosphorylated form of Receptor-Interacting Serine/Threonine-Protein Kinase 1 (RIPK1), a key regulator of TNF-mediated apoptosis, necroptosis, and inflammatory pathways. This protein consists of an N-terminal kinase domain, an intermediate domain containing the RIP homotypic interaction motif (RHIM), and a C-terminal death domain. When phosphorylated at Ser166, RIPK1 is involved in the formation of necrosis-inducing complexes and is considered a biomarker for the activation of RIPK1 kinase and necroptosis. This phosphorylation can be effectively inhibited by Nec-1, a necroptosis inhibitor.
免疫印迹
WB result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with RIP-Myc-His fusion protein, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 85 kDa
斑点杂交
Dot blot result of Phospho-RIP (Ser166) Recombinant Rabbit mAb
Lane 1: RIP (Ser166) phospho peptide 1
lane 2: RIP (Ser166) phospho peptide 2
lane 3: RIP (Ser166) unmodified peptide 1
lane 4: RIP (Ser166) unmodified peptide 2
Primary antibody: Phospho-RIP (Ser166) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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