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Rabbit抗原名称
RNF40分子别名
E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein (RBP95); RING finger protein 40; RING-type E3 ubiquitin transferase BRE1B; BRE1B; KIAA0661免疫原
Recombinant Protein细胞定位
NucleusAccession
O75150克隆号
S-1068-279抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, HeLa, HCT 116, HepG2, RAW264.7, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
RNF40 (Ring Finger Protein 40) is an E3 ubiquitin ligase that plays a critical role in histone H2B monoubiquitination at lysine 120 (H2Bub1), which is essential for gene regulation and chromatin remodeling. This protein is involved in various biological processes, including DNA damage repair, apoptosis, and cancer development. Recent studies have highlighted its dual roles in cancer progression. For example, in triple-negative breast cancer (TNBC), RNF40 supports cancer stem cell properties and glycolytic programs, promoting tumor aggressiveness. Additionally, RNF40 regulates lipid metabolism by targeting LIMA1 for proteasome-mediated degradation, thereby influencing cellular sensitivity to lipid synthesis inhibitors. These findings underscore RNF40's multifaceted roles in epigenetic regulation and cancer biology, making it a potential therapeutic target.
免疫印迹
WB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDaWB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDaWB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of RNF40 in tumor tissue.
Expression of RNF40 in human tissue.
Expression of RNF40 in mouse & rat tissue.
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