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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H3 (acetyl K4) Recombinant Rabbit mAb (S-848-137)

H3K4ac

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1263
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (acetyl K4)

  • 分子别名

    H3K4ac

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-848-137

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    乙酰化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:250-1:1000 Hu
    ICC 1:500 Hu
背景介绍

  • Histone H3 (acetyl K4) refers to the acetylation of lysine 4 on the histone H3 protein, which is a post-translational modification that plays a critical role in gene regulation. This modification is associated with active transcription, as it weakens the interaction between histones and DNA, leading to a more open chromatin structure that is accessible to the transcription machinery. Histone H3 acetylation at lysine 4 (H3K4ac) is enriched at the transcription start sites (TSSs) of active genes and enhancers, contributing to transcription by promoting H3K4 trimethylation (H3K4me3) and facilitating histone eviction. Moreover, H3K4ac is positively correlated with H3K4 methylation, and both modifications are important for marking active chromatin regions that dictate transgene expression or silencing. The dynamic turnover of acetylation on K4-methylated histone H3 is also significant for efficient gene induction, as inhibition of this turnover can lead to immediate inhibition of gene expression. In summary, Histone H3 (acetyl K4) is a key epigenetic marker that influences gene transcription and chromatin structure.

  • 免疫印迹

    • WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 30 mM Sodium butyrate for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 500ng/mL TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 500ng/mL TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 斑点杂交

    • Dot blot result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
      Lane 1: Histone H3K4 acetyl peptide
      Lane 2: Histone H3 unmodified peptide
      Lane 3: Histone H3K4 methyl peptide
      Lane 4: Histone H3K9 acetyl peptide
      Lane 5: Histone H3K9 methyl peptide
      Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with Sodium butyrate (30mM, 4hr) (top panel) and untreated HeLa cells (below panel). Anti- Histone H3 (acetyl K4) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Histone H3 (acetyl K4) in tumor tissue.

    • Expression of Histone H3 (acetyl K4) in human tissue.

    • Expression of Histone H3 (acetyl K4) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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