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宿主来源
Rabbit抗原名称
NDUFA1分子别名
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; Complex I-MWFE (CI-MWFE); NADH-ubiquinone oxidoreductase MWFE subunit免疫原
Synthetic PeptideAccession
O15239克隆号
S-1277-177抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, HT-1080, A549, mouse brain, mouse heart, mouse skeletal muscle, rat brain, rat heart, rat skeletal muscle预测反应种属
(反应种属缩写表)Cz纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:500 Hu, Ms, Rt ICC 1:100 Hu
NDUFA1, or NADH:ubiquinone oxidoreductase subunit A1, is an essential component of Complex I in the mitochondrial respiratory chain, which plays a critical role in transferring electrons from NADH to ubiquinone. NDUFA1 shows ubiquitous expression across various tissues, with particularly high levels in the kidney and colon, indicating its fundamental role in cellular respiration. Research has indicated that NDUFA1 may have prognostic significance in certain types of cancer, reflecting its role in tumor metabolism and progression. NDUFA1 has also been shown to play a protective role in the heart by enhancing mitochondrial bioenergetics and coordinating the assembly of respiratory complexes and supercomplexes, which can protect against ischemia-reperfusion injury.
免疫印迹
WB result of NDUFA1 Recombinant Rabbit mAb
Primary antibody: NDUFA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 8 kDa
Observed MW: 12 kDaWB result of NDUFA1 Recombinant Rabbit mAb
Primary antibody: NDUFA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 8 kDa
Observed MW: 12 kDaWB result of NDUFA1 Recombinant Rabbit mAb
Primary antibody: NDUFA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat heart lysate 20 µg
Lane 3: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 8 kDa
Observed MW: 12 kDa
流式分析
免疫沉淀
NDUFA1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating NDUFA1 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using NDUFA1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: NDUFA1 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 10 kDa
Observed MW: 12 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-NDUFA1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- NDUFA1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of NDUFA1 in tumor tissue.
Expression of NDUFA1 in human tissue.
Expression of NDUFA1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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