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Histone H3 (acetyl K18) Recombinant Rabbit mAb (S-1688-90)

H3K18ac

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1154
规格
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (acetyl K18)

  • 分子别名

    H3K18ac

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-1688-90

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    乙酰化

  • 应用

    ChIP ,
    IHC-P ? ,
    ICC ? ,
    WB ,
    IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Ar, C.el, Dr, Hm, Or, Pl, Ys

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
Dot Blot 1:1000
WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu
ChIP 1:20-1:50 Hu
背景介绍

  • Histone H3 (acetyl K18) refers to the specific acetylation of lysine 18 on the histone protein H3. Acetylation of histone proteins, including H3K18, is a type of epigenetic mark that can influence gene expression without altering the DNA sequence itself. This modification neutralizes the positive charge of lysine residues, which can weaken the interaction between histones and DNA, leading to changes in chromatin structure and accessibility of DNA to the transcription machinery. Histone H3 acetylation at K18 is associated with transcriptional activation. It is one of the modifications that can lead to a more open chromatin state, facilitating access to DNA for transcription factors and the RNA polymerase complex.

  • 免疫印迹

    • WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 1 µM TSA for 18 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C6 whole cell lysate 20 µg
      Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 免疫沉淀

    • Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H3 (acetyl K18) in 0.4 mg HeLa+TSA(1 μM, 18 hr) whole cell lysate.
      Western blot was performed on the immunoprecipitate using Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa+TSA(1 μM, 18 hr) whole cell lysate 20 µg (Input)
      Lane 2: Histone H3 (acetyl K18) Recombinant Rabbit mAb IP in HeLa+TSA(1 μM, 18 hr) whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa+TSA(1 μM, 18 hr) whole cell lysate
      Predicted MW: 15 kDa
      Observed MW: 17 kDa
      Exposure time: 10 s

  • 斑点杂交

    • Dot blot result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
      Lane 1: H3K18ac modified peptide
      Lane 2: H3K18 unmodified peptide
      Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K18) Recombinant Rabbit mAb (S-1688-90) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in Histone H3 (acetyl K18) Recombinant
      Rabbit mAb (S-1688-90)-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of Histone H3 (acetyl K18) in tumor tissue.

    • Expression of Histone H3 (acetyl K18) in human tissue.

    • Expression of Histone H3 (acetyl K18) in mouse & rat tissue.

  • 斯达特公司的抗体，可以回收利用几次？

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