Caspr Recombinant Rabbit mAb (S-1706-1)

Contactin-associated protein 1,Caspr1,Neurexin IV,Neurexin-4,p190,CNTNAP1,NRXN4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1125

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Caspr
分子别名

Contactin-associated protein 1; Caspr1; Neurexin IV; Neurexin-4; p190; CNTNAP1; NRXN4
免疫原

Synthetic Peptide
细胞定位

Membrane, Cell junction
Accession

P78357
克隆号

S-1706-1
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, SH-SY5Y, U-87 MG, IMR-32, Neuro-2a, PC-12, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

IHC-P ? ,
WB ,
IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
IF 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
IF	1:200	Hu, Ms, Rt

背景介绍

Caspr, also known as contactin-associated protein (CNTNAP), is a transmembrane protein that plays a crucial role in the nervous system. It is particularly important for the formation and maintenance of the myelin sheath in both the central and peripheral nervous systems. Caspr is expressed at high levels in the paranodal regions of myelinated axons, where it contributes to the formation of septate-like junctions between axons and myelinating cells. Caspr is also essential for the proper localization of voltage-gated potassium channels (VGKC) in the nervous system. It helps in the aggregation and development of K channels (Kv1.1 and Kv1.2) in the proximal nodal region, which is crucial for axon formation and stability. Furthermore, Caspr is a component of the paranodal junctions, which are critical for maintaining the integrity of myelinated axons. It helps in the reciprocal signaling between axons and glial cells, ensuring proper axonal function.

免疫印迹
- WB result of Caspr Recombinant Rabbit mAb
  Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Lane 3: U-87 MG whole cell lysate 20 µg
  Lane 4: IMR-32 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 156 kDa
  Observed MW: 200 kDa
- WB result of Caspr Recombinant Rabbit mAb
  Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 156 kDa
  Observed MW: 200 kDa
- WB result of Caspr Recombinant Rabbit mAb
  Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 156 kDa
  Observed MW: 200 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of Caspr in human tissue.
- Expression of Caspr in mouse & rat tissue.