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Mouse抗原名称
HLA-ABC分子别名
HLA class I histocompatibility antigen; A alpha chain; Human leukocyte antigen A (HLA-A); HLA class I histocompatibility antigen; B alpha chain; Human leukocyte antigen B (HLA-B); HLA class I histocompatibility antigen, C alpha chain; HLA-Cw; Human leukocyte antigen C细胞定位
Cell membraneAccession
P04439, P01889, P10321克隆号
S-R526抗体类型
Mouse mAb抗体同种型
IgG2a,k应用
FCM, ICC反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:100 | Hu |
| FCM | 1:2000 | Hu |
HLA-A,BC are part of the Human Leukocyte Antigen (HLA) system, which is the human version of the Major Histocompatibility Complex (MHC). HLA-ABC are classic Class I molecules of the HLA system, expressed on the surface of all nucleated cells. They are responsible for presenting endogenous antigens, such as those produced by viruses or tumor cells, to CD8+ T cells, thereby activating cellular immune responses. These molecules consist of a heavy chain and a light chain (β2-microglobulin), with the heavy chain encoded by the HLA-ABC genes. In medical research, the expression of HLA-A,BC has been studied in various diseases, especially in tumor immunology. Research on HLA-ABC also includes the frequency and clinical significance of their haplotypes in haplotype transplantation. Studying the frequency of these haplotypes is valuable for selecting the best donors and unrelated donors, particularly when performing related HLA haplotype transplantations.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling human HLA-ABC antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HL-60 cells (top panel) and negative staining in K562 cells (below panel). Anti-human HLA-ABC antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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