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别名
COL6A1,Collagen alpha-1(VI) chain宿主来源
Rabbit抗原名称
Collagen VI (COL6A1)免疫原
Recombinant Protein细胞定位
Secreted, Extracellular matrixAccession
P12109克隆号
S-1495-18抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Hu阳性样本
PC-3, WI-38纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| IF | 1:250 | Hu |
Collagen Type VI, which includes the alpha 1 chain encoded by the COL6A1 gene, is an extracellular matrix protein that plays a crucial role in maintaining the integrity and function of muscles and skin. It is a microfibrillar protein that forms a complex with other collagen types, particularly Type I collagen, to provide structural support and tensile strength to connective tissues. The COL6A1 gene is responsible for encoding the alpha 1 chain of collagen VI, and mutations in this gene can lead to a spectrum of muscular disorders known as collagen VI-related myopathies, such as Ullrich congenital muscular dystrophy and Bethlem myopathy. These conditions are characterized by muscle weakness and stiffness, and in some cases, additional symptoms like joint contractures and skin abnormalities. Research has also implicated collagen VI in various pathological processes beyond myopathies. For instance, it has been suggested that COL6A1 may play a role in the progression of certain cancers, including osteosarcoma, by promoting cell migration and invasion3. Additionally, alterations in COL6A1 expression have been associated with tumorigenesis and angiogenesis in glioblastoma multiforme.
免疫印迹
WB result of Collagen VI (COL6A1) Recombinant Rabbit mAb
Primary antibody: Collagen VI (COL6A1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: WI-38 whole cell lysate 20 µg
Negative control: HT-29 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 109 kDa
Observed MW: 130 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human prostate. Anti-Collagen VI (COL6A1) antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Collagen VI (COL6A1) in tumor tissue.
Expression of Collagen VI (COL6A1) in human tissue.
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