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Rabbit抗原名称
Phospho-S6 Ribosomal Protein (Ser240/244)分子别名
Small ribosomal subunit protein eS6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6细胞定位
CytoplasmAccession
P62753克隆号
S-R248抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC, WB, IP反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| ICC | 1:500 |
Phospho-S6 Ribosomal Protein (Ser240/244) refers to the phosphorylated form of the S6 ribosomal protein at serine residues 240 and 244. This post-translational modification is crucial for the protein's role in regulating protein synthesis and cell growth. The phosphorylation of S6 is often induced by various growth factors and is mediated by the mammalian target of rapamycin (mTOR) through its downstream effector, the ribosomal protein S6 kinase (S6K). In the context of cell signaling, Phospho-S6 Ribosomal Protein (Ser240/244) serves as a marker for the activation of the mTOR pathway, which is involved in various cellular processes, including cell proliferation, metabolism, and survival. The mTOR pathway is frequently deregulated in cancer, making Phospho-S6 a potential biomarker for cancer diagnosis and prognosis, as well as a target for therapeutic interventions. Moreover, the level of phospho-S6 ribosomal protein has been correlated with the efficacy of mTOR inhibitors in clinical trials, suggesting its potential as a predictive marker for response to therapy.
免疫印迹
WB result of Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb
Primary antibody: Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 grown in serum-free media overnight whole cell lysate 20 µg
Lane 2: NIH/3T3 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDaWB result of Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb
Primary antibody: Phospho-S6 Ribosomal Protein (Ser240/244) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 grown in serum-free media overnight whole cell lysate 20 µg
Lane 2: PC-12 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 34 kDa
免疫沉淀
Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-S6 Ribosomal Protein (Ser240/244) in 0.4 mg NIH/3T3 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: NIH/3T3 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb IP in NIH/3T3 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes whole cell lysate
Predicted MW: 29 kDa
Observed MW: 32 kDa
免疫细胞化学
ICC analysis of NIH/3T3 cells (grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes, top panel) and NIH/3T3 cells (grown in serum-free media overnight, below panel). Anti- Phospho-S6 Ribosomal Protein (Ser240/244) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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