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Rabbit抗原名称
sFlt-1/VEGF Receptor 1分子别名
VEGFR-1, FRT免疫原
Synthetic Peptide细胞定位
SecretedAccession
P17948克隆号
S-807-137抗体类型
Recombinant mAb抗体同种型
IgG应用
WB, IP反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
Vascular endothelial growth factor (VEGF) represents a growth factor with important pro-angiogenic activity, having a mitogenic and an anti-apoptotic effect on endothelial cells, increasing the vascular permeability, promoting cell migration, etc. VEGF binds to tyrosine kinase cell receptors (VEGFRs): VEGFR-1 [Fms-like tyrosine kinase 1 (Flt-1)], VEGFR-2 [kinase insert domain receptor (KDR) in human; fetal liver kinase 1 (Flk-1) in mouse] and VEGFR-3 [Fms-like tyrosine kinase 4 (Flt-4)]. While VEGFR-1 and VEGFR-2 are expressed predominantly on vascular endothelial cells, VEGFR-3 is expressed especially on lymphatic endothelial cells.
免疫印迹
WB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HUVEC whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDaWB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
免疫沉淀
sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating sFlt-1/VEGF Receptor 1 in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 whole cell lysate 10 µg (Input)
Lane 2: sFlt-1/VEGF Receptor 1 Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 150 kDa
Observed MW: 180 kDa
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