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宿主来源
Rabbit抗原名称
K-ε-GG免疫原
Synthetic Peptide抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Dot blot
Immunoaffinity purification
稀释度
应用 稀释度 Dot Blot 1：1000
The K-ε-GG motif refers to the diglycine remnant left on the lysine residue of a protein after ubiquitination, a form of post-translational modification (PTM). In proteomics research, the analysis of the K-ε-GG motif typically involves the use of specific antibodies to enrich these modified peptides, followed by identification and quantification using mass spectrometry techniques. This analytical approach helps researchers better understand the role of ubiquitination in biological processes and how it changes under disease conditions. Furthermore, antibodies against the K-ε-GG motif are also used in ubiquitination proteomics technology, which combines immunoaffinity enrichment with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for large-scale qualitative and quantitative analysis of ubiquitinated proteins.
斑点杂交
Dot blot result of K-ε-GG Rabbit pAb
Lane 1: Modified peptide library ((K-ε-GG)
Lane 2: Modified peptide library ((K-ε-Gv)
Lane 3: Nnmodified peptide library (K)
Primary antibody: K-ε-GG Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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