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Rabbit抗原名称
DAZL分子别名
Deleted in azoospermia-like, DAZ-like autosomal, Deleted in azoospermia-like 1, Dazl1, Dazla免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q64368克隆号
S-1552-6抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:2000 |
| IF | 1:2000 |
DAZL, or Deleted in Azoospermia-Like, is an RNA-binding protein that plays a critical role in germ cell development and maturation. It is highly expressed in primordial germ cells (PGCs) and is essential for their proper development and function. DAZL has been shown to regulate gene expression at both the transcriptional and post-transcriptional levels. It interacts with specific sequences in the 3' untranslated regions (UTRs) of target mRNAs, thereby influencing their stability, translation, and ultimately, their contribution to the germ cell's proteome. Recent research has uncovered additional layers of DAZL's functionality. It can also bind to microRNA (miRNA) precursors and enhance the processing activity of Dicer, an enzyme crucial for miRNA maturation. This discovery expands the known mechanisms by which DAZL can modulate gene expression and suggests that it may have a broader role in regulating cellular processes than previously understood.
免疫印迹
WB result of DAZL Recombinant Rabbit mAb
Primary antibody: DAZL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDaWB result of DAZL Recombinant Rabbit mAb
Primary antibody: DAZL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-DAZL antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DAZL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-DAZL antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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