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PI3 Kinase p110β Recombinant Rabbit mAb (S-1193-109)

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform,PI3-kinase subunit beta,PI3K-beta,PI3Kbeta,PtdIns-3-kinase subunit beta,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta,PtdIns-3-kinase subunit p110-beta,p110beta,Serine/threonine protein kinase PIK3CB,PIK3CB,PIK3C1

货号： S0B0904

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, Serine/threonine protein kinase PIK3CB, PIK3CB, PIK3C1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P42338

  • 克隆号

    S-1193-109

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
ICC 1:200
背景介绍

  • PI3 Kinase p110 β, also known as PIK3CB, is a catalytic subunit of the phosphoinositide 3-kinase (PI3K) family, which plays a critical role in cellular processes such as growth, metabolism, and survival. PI3Ks are enzymes that generate phosphoinositol lipids as second messengers, initiating multiple signal transduction cascades. The p110 β isoform has been shown to have distinct functions in different cellular contexts and contributes to cell growth and development, DNA replication, insulin sensitivity, tumorigenesis, and G-protein-coupled receptor-activated signaling.

  • 免疫印迹

    • WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
      Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: K562 whole cell lysate 40 µg
      Lane 2: MCF7 whole cell lysate 40 µg
      Lane 3: HeLa whole cell lysate 40 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 123 kDa
      Observed MW: 100~130 kDa

    • WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
      Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C2C12 whole cell lysate 40 µg
      Lane 2: mouse brain lysate 40 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 123 kDa
      Observed MW: 110~150 kDa

    • WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
      Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 40 µg
      Lane 2: rat brain lysate 40 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 123 kDa
      Observed MW: 110~150 kDa

  • 免疫细胞化学

    • ICC shows positive staining in C2C12 cells. Anti- PI3 Kinase p110 β antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p110 β antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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