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宿主来源
Rabbit抗原名称
VISTA分子别名
V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; Stress-induced secreted protein-1 (Sisp-1); V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; C10orf54; SISP1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q9H7M9克隆号
S-1469-72抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, FCM, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| IHC-P | 1:250 |
| FCM | 1:50 |
VISTA is an important immune modulatory receptor that is gaining attention in cancer immunotherapy. VISTA exerts its suppressive effects on T cell activity by binding to their respective ligands on T cells, potentially influencing the immune evasion mechanisms of tumors. Studies have shown that VISTA is expressed in a variety of cancers, including melanoma, pancreatic cancer, and prostate cancer, and its expression levels are closely related to the immunosuppressive state of tumors.
免疫印迹
WB result of VISTA Recombinant Rabbit mAb
Primary antibody: VISTA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: DU 145 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40~55 kDa
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling VISTA antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD14 - Alexa Fluor® 488 separately. Gated on total viable cells.
免疫沉淀
VISTA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VISTA in 0.4 mg DU 145 whole cell lysate.
Western blot was performed on the immunoprecipitate using VISTA Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: DU 145 whole cell lysate 20 µg (Input)
Lane 2: VISTA Rabbit mAb IP in DU 145 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in DU 145 whole cell lysate
Predicted MW: 34 kDa
Observed MW: 40~55 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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