Histone H3 (Crotonyl K9) Recombinant Rabbit mAb (S-R409)

H3K9Cr

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0879

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (Crotonyl K9)
分子别名

H3K9Cr
细胞定位

Nucleus
Accession

P68431
克隆号

S-R409
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

巴豆酰化
反应种属 ?

Hu
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:500-1:1000
IP 1:100
ICC 1:1000
ChIP 1:20-1:50

应用	稀释度
WB	1:500-1:1000
IP	1:100
ICC	1:1000
ChIP	1:20-1:50

背景介绍

Histone H3 crotonylation, specifically at lysine 9 (H3K9cr), is a form of post-translational modification that has been identified as a key player in epigenetic regulation, particularly in plants 。This modification is structurally and functionally distinct from the well-studied lysine acetylation and is involved in various biological processes, including chromatin remodeling, metabolism, cell cycle, and cellular organization. In the context of histones, crotonylation can be found on different lysine residues among plant species and is known to overlap with the histone modification H3K9ac in about 95% of the regions, which suggests a potential interplay between these two modifications.

免疫印迹
- WB result of Histone H3 (Crotonyl K9) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (Crotonyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
  This blot was developed with high sensitivity substrate
免疫沉淀
- Histone H3 (Crotonyl K9) Rabbit mAb at 1/100 dilution (1 µg) immunoprecipitating Histone H3 (Crotonyl K9) in 0.4 mg HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate.
  Western blot was performed on the immunoprecipitate using Histone H3 (Crotonyl K9) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg (Input)
  Lane 2: Histone H3 (Crotonyl K9) Rabbit mAb IP in HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti-Histone H3 (Crotonyl K9) antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+TSA (500ng/ml,4h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (Crotonyl K9) Recombinant Rabbit mAb (S-R409) and Rabbit mAb IgG Isotype Control (SDT-R173) only at 1:20 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (Crotonyl K9)
  Recombinant Rabbit mAb (S-R409)-immunoprecipitated
  sample.