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宿主来源
Rabbit抗原名称
His tag免疫原
Synthetic Peptide抗体同种型
IgG反应种属 ?
Species Independent纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM ,ICC ? ,WB稀释度
应用 稀释度 WB 1:5000 ICC 1:500-1:2000 ICFCM 1:5000
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
免疫印迹
WB result of His tag Rabbit pAb
Primary antibody: His tag Rabbit pAb at 1/5000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T transfected with myc-his tag whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 20 kDaWB result of His tag Rabbit pAb
Primary antibody: His tag Rabbit pAb at 1/5000 dilution
Lane 1: Angiopoietin-1 (277-498) His Tag Protein, Mouse 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28~33 kDa
Observed MW: 28 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Myc, His transfected 293T (Human embryonic kidney epithelial cell) labelling His tag antibody at 1/5000 (0.01 μg) dilution/ (right panel) compared with a Rabbit IgG Isotype Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in Histone H3-His Tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti-His tag Rabbit Polyclonal antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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