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宿主来源
Rabbit抗原名称
Histone H3 (Crotonyl K18 + K23)细胞定位
NucleusAccession
P68431克隆号
S-R410抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
巴豆酰化反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500 Hu, Ms ICC 1:500 Hu, Ms ChIP 1:20-1:50 Hu
Histone H3 (Crotonyl K18 + K23)" refers to Histone H3 that has undergone a particular type of PTM known as crotonylation at two specific lysine residues: lysine 18 (K18) and lysine 23 (K23). Crotonylation is a type of acylation, where an acyl group (in this case, a crotonyl group, derived from crotonic acid) is added to a lysine residue on the histone protein. This modification is thought to have regulatory functions similar to other acylations, such as acetylation, which are often associated with active gene transcription. Crotonylation of histone H3 at K18 and K23 may alter the interaction of histones with DNA and other chromatin-associated proteins, thereby influencing chromatin structure and accessibility to transcription factors and other regulatory complexes.
免疫印迹
WB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 30 mM Sodium Butyrate for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Histone H3 (Crotonyl K18 + K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (Crotonyl K18 + K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + TSA (500ng/ml,4h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb (S-R410) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment
of RPL30, GAPDH, MYOD1, AFM, SAT-α and SAT-2 in Histone H3 (Crotonyl
K18 + K23) Recombinant Rabbit mAb (S-R410)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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