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Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb (S-R410)

货号： S0B0853

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (Crotonyl K18 + K23)

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-R410

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:500
ICC 1:500
背景介绍

  • Histone H3 (Crotonyl K18 + K23)" refers to Histone H3 that has undergone a particular type of PTM known as crotonylation at two specific lysine residues: lysine 18 (K18) and lysine 23 (K23). Crotonylation is a type of acylation, where an acyl group (in this case, a crotonyl group, derived from crotonic acid) is added to a lysine residue on the histone protein. This modification is thought to have regulatory functions similar to other acylations, such as acetylation, which are often associated with active gene transcription. Crotonylation of histone H3 at K18 and K23 may alter the interaction of histones with DNA and other chromatin-associated proteins, thereby influencing chromatin structure and accessibility to transcription factors and other regulatory complexes.

  • 免疫印迹

    • WB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 30 mM Sodium Butyrate for 4 hours whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (Crotonyl K18 + K23) Recombinant Rabbit mAb at 1/500 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Histone H3 (Crotonyl K18 + K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- Histone H3 (Crotonyl K18 + K23) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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