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NDUFS8 Recombinant Rabbit mAb (S-1273-10)

Mitochondrial NADH dehydrogenase [ubiquinone] iron-sulfur protein 8,Complex I-23kD (CI-23kD),NADH-ubiquinone oxidoreductase 23 kDa subunit,TYKY subunit

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0813

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

NDUFS8
分子别名

Mitochondrial NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, Complex I-23kD (CI-23kD), NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit
免疫原

Synthetic Peptide
Accession

O00217
克隆号

S-1273-10
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Gor
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:100-1:200
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:100-1:200
IP	1:50

背景介绍

The NDUFS8 protein is a subunit of NADH dehydrogenase (ubiquinone) also known as Complex I, which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Mutations in NDUFS8 have been associated with mitochondrial diseases, which can cause any one of a clinically heterogeneous group of disorders arising from dysfunction of the mitochondrial respiratory chain. The phenotypic spectrum ranges from isolated diseases affecting single organs to severe multisystem disorders. NDUFS8 mutations have also been associated with Leigh syndrome.

免疫印迹
- WB result of NDUFS8 Recombinant Rabbit mAb
  Primary antibody: NDUFS8 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
免疫沉淀
- NDUFS8 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating NDUFS8 in 0.4 mg HepG2 whole cell lysate.
  Western blot was performed on the immunoprecipitate using NDUFS8 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HepG2 whole cell lysate 20 µg (Input)
  Lane 2: NDUFS8 Rabbit mAb IP in HepG2 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
  Predicted MW: 23 kDa
  Observed MW: 23 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NDUFS8 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-NDUFS8 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-NDUFS8 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NDUFS8 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.