热搜词：

CN

  • CN
  • EN

注册

产品中心 抗体 其他一抗

所有图片（1/9）

PKC zeta Recombinant Rabbit mAb (S-1306-34)

Protein kinase C zeta type,nPKC-zeta

货号： S0B0802

Datasheet COA
  • 价格： ￥260
  • 规格：
  • 数量：
申请试用 斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PKC zeta

  • 分子别名

    Protein kinase C zeta type; nPKC-zeta

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Membrane

  • Accession

    P09217

  • 克隆号

    S-1306-34

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ICFCM, IHC-P, ICC, WB, IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
IHC-P 1:200
ICC 1:500
ICFCM 1:500
背景介绍

  • Protein kinase C, zeta (PKCζ), also known as PRKCZ, is a protein in humans that is encoded by the PRKCZ gene. The PRKCZ gene encodes at least two alternative transcripts, the full-length PKCζ and an N-terminal truncated form PKMζ. PKMζ is thought to be responsible for maintaining long-term memories in the brain. Like other PKC isoforms, PKCζ is a serine/threonine kinase that adds phosphate groups to target proteins. It is atypical in that unlike other PKC isoforms, PKCζ does not require calcium or diacylglycerol (DAG) to become active, but rather relies on a different second messenger, presumably generated through a phosphoinositide 3-kinase (PI3-kinase) pathway.

  • 免疫印迹

    • WB result of PKC zeta Recombinant Rabbit mAb
      Primary antibody: PKC zeta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: MDA-MB-231 whole cell lysate 20 µg
      Lane 2: HT-29 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 68 kDa
      Observed MW: 70 kDa

    • WB result of PKC zeta Recombinant Rabbit mAb
      Primary antibody: PKC zeta Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse lung lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 68 kDa
      Observed MW: 70 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling PKC zeta antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • PKC zeta Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PKC zeta in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using PKC zeta Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: PKC zeta Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 68 kDa
      Observed MW: 70 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PKC zeta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PKC zeta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PKC zeta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-PKC zeta antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- PKC zeta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    评论(0)