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Mouse克隆号
C1.18.4抗体类型
Mouse mAb抗体同种型
Mouse IgG2a, κ应用
In vivo control, FCM, ELISA纯化方式
Protein G浓度
5 mg/ml纯度
>95% Determined by SDS-PAGE内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 |
|---|---|
| ICFCM | 1:200 |
| FCM | 1:200 |
Isotype control antibodies, to estimate the nonspecific binding of target.
流式分析
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (Human cervix adenocarcinoma epithelial cell) labelling mouse IgG2a isotype control at 1/200 (1 μg) dilution (Left) / (Red) compared with α-tubulin antibody (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling mouse IgG2a isotype control at 1/200 (1 μg) dilution (Left) / (Red) compared with CD13 antibody at 1/200 (1 μg) dilution (S0B0441) (Right) / (Red), Mouse monoclonal IgG Isotype Control (Left) / (Black) compared with mouse IgG2a isotype control (Right) / (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
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