Histone H3 (Lactyl K9) Recombinant Rabbit mAb (S-R397)

H3K9lac

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0756

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (Lactyl K9)
分子别名

H3K9lac
细胞定位

Nucleus
Accession

P68431
克隆号

S-R397
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乳酸化
应用

ChIP ,
ICC ? ,
WB ,
IP
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50
ICC	1:500
ChIP	1:20-1:50

背景介绍

H3K9lac refers to the lactylation modification that occurs at the 9th lysine residue of histone H3. This modification is a relatively novel histone modification discovered in recent epigenetic studies. This modification may alter the interaction between histones, DNA, and other proteins, thereby affecting gene expression and cellular functions.

免疫印迹
- WB result of Histone H3 (Lactyl K9) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (Lactyl K9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
免疫沉淀
- Histone H3 (Lactyl K9) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H3 (Lactyl K9) in 0.4 mg HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate.
  Western blot was performed on the immunoprecipitate using Histone H3 (Lactyl K9) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg (Input)
  Lane 2: Histone H3 (Lactyl K9) Rabbit mAb IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
免疫细胞化学
- ICC analysis of HeLa cells treated with sodium lactate (100 mM, 24 hours) (top panel) and HeLa cells untreated with sodium lactate (100 mM, 24 hours) (below panel). Anti- Histone H3 (Lactyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells + Lactate sodium (100 mM, 24 hours) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (Lactyl K9) Recombinant Rabbit mAb (S-R397) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (Lactyl K9) Recombinant
  Rabbit mAb (S-R397)-immunoprecipitated sample.