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Rabbit抗原名称
Histone H3 (acetyl K14)分子别名
H3K14ac细胞定位
NucleusAccession
P68431克隆号
S-R398抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms ICC 1:500 Hu ChIP 1:20-1:50 Hu
H3K14ac is a post-translational modification form of histone H3, specifically referring to the acetylation of its 14th lysine residue. The modification of H3K14ac is closely related to gene transcription regulation. Acetylation modifications are generally associated with the activation of gene transcription, as they can alter the chromatin structure, making DNA more accessible to transcription factors and RNA polymerases. In certain studies, such as the research on novel products of Akkermansia muciniphila, the upregulation of H3K14ac promotes the transcription and secretion of heat shock protein 70 (HSP70) in cancer cells, thereby influencing the tumor microenvironment. Abnormal modifications of H3K14ac may be related to the occurrence and development of various diseases. For instance, in epithelial ovarian tumors, the expression of H3K14Ac may be associated with the histological grade and clinical stage of ovarian cancer. In studies on arsenic poisoning, H3K14ac is linked to arsenic poisoning and arsenic-induced early toxic effects, potentially providing an effective biomarker for early monitoring of arsenic poisoning.
免疫印迹
WB result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
免疫细胞化学
ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti- Histone H3 (acetyl K14) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,17h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-R398) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, GAPDH, MYOD1, AFM, SAT-α and SAT-2 in Histone H3 (acetyl K14)
Recombinant Rabbit mAb (S-R398)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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