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Rabbit抗体类型
Polyclonal antibody抗体同种型
IgG应用
WB, IP反应种属 ?
Species Independent纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000-1:2000 |
| IP | 1:50 |
Lysine lactylation is an emerging post-translational modification that occurs in cellular metabolism, specifically referring to the accumulation of lactic acid modifying lysine residues on histones. Lysine lactylation modifies the chromatin structure, thereby affecting gene expression. Research has found that lactylation sites are widely distributed in gene promoter regions, closely associated with gene transcriptional activation. Lysine lactylation also participates in energy metabolism processes, potentially related to the dynamic balance regulation of histone lysine acetylation.
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WB result of L-Lactyl Lysine Rabbit pAb
Primary antibody: L-Lactyl Lysine Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
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L-Lactyl Lysine Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating L-Lactyl Lysine in 0.4 mg HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate.
Western blot was performed on the immunoprecipitate using L-Lactyl Lysine Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate 20 µg (Input)
Lane 2: L-Lactyl Lysine Rabbit pAb IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 mM Lactate sodium for 24 hours whole cell lysate
Predicted MW: Multiple
Observed MW: Multiple
引用文献(1)
- Discovery of protein lactylation-associated biomarkers and their potential pathogenic mechanisms in recurrent spontaneous abortion
X Yan, X Wang, J Jiao, X Sun
International Journal of Biological Macromolecules. 2025 Sep 01 .
影响因子： 7.7
货号：S0B0719产品名称：L-Lactyl Lysine Rabbit Polyclonal Antibody
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