Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb (S-748-20)

Glycogen synthase kinase-3 beta,Serine/threonine-protein kinase GSK3B,GSK3B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0518

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
分子别名

Glycogen synthase kinase-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P49841
克隆号

S-748-20
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Ms, Rt, Sq, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20°C as supplied
应用

WB

IP
稀释度

应用稀释度

Dot Blot 1:1000
WB 1:1000
IP 1:50

应用	稀释度
Dot Blot	1:1000
WB	1:1000
IP	1:50

背景介绍

GSK-3 is a serine/threonine protein kinase that phosphorylate either threonine or serine, and this phosphorylation controls a variety of biological activities, such as glycogen metabolism, cell signaling, cellular transport, and others. GSK-3 has also been shown to regulate immune and migratory processes. GSK-3 participates in a number of signaling pathways in the innate immune response, including pro-inflammatory cytokine and interleukin production. GSK-3 is also integrally tied to pathways of cell proliferation and apoptosis. GSK-3 has been shown to phosphorylate Beta-catenin, thus targeting it for degradation. GSK-3 is therefore a part of the canonical Beta-catenin/Wnt pathway, which signals the cell to divide and proliferate. Insulin indirectly inactivates GSK3 via downstream phosphorylation of the specific serine residues Ser21 and Ser9 in GSK-3 isoforms α and β, respectively, via the PI3K/Akt pathway (protein kinase B).

免疫印迹
- WB result of Phospho-GSK-3β (Ser9) Rabbit mAb
  Primary antibody: Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HeLa starve overnight , then treated with Calyculin A (100nM, 30 min) whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 47 kDa
  Observed MW: 45 kDa
  Exposure time: 180 s
- WB result of Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb
  Primary antibody: Phospho-GSK-3β (Ser9) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve for 3hr, then treated with Calyculin A (100nM/ml, 30min) whole cell lysate 20 µg
  Negative control: Untreated NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 47 kDa
  Observed MW: 50kDa
免疫沉淀
- Phospho-GSK-3β (Ser9) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-GSK-3β (Ser9) in 0.4 mg HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate 20 µg (Input)
  Lane 2: Phospho-GSK-3β (Ser9) Rabbit mAb IP in HeLa starve overnight, then treated with
  Calyculin A (100nM) for 30 minutes whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa starve overnight, then treated with Calyculin A (100nM) for 30 minutes whole cell lysate
  Predicted MW: 47 kDa
  Observed MW: 47 kDa
  Exposure time: 30 s（This blot was developed with high sensitivity substrate）
斑点杂交
- Dot blot result of Phospho-GSK-3β (Ser9) Rabbit mAb
  Lane 1: Phospho-GSK-3β (Ser9) peptide
  Lane 2: Phospho-GSK-3β peptide
  Primary antibody: Phospho-GSK-3β (Ser9) Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Exposure time: 18 s