CD3d Recombinant Rabbit mAb (S-R318)

CD3 Delta,T-cell surface glycoprotein CD3 delta chain,T-cell receptor T3 delta chain,T3D

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0440

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

CD3d
分子别名

CD3 Delta, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, T3D
细胞定位

Cell membrane
Accession

P04234
克隆号

S-R318
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:250
ICC 1:500
ICFCM 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:250
ICC	1:500
ICFCM	1:500

背景介绍

CD3D is one of the components of the T-cell receptor (TCR)/CD3 complex and functions in the signal transduction of T-cell activation. A TCR-mediated signal is conducted across the plasmalemma through the CD3 chain upon extracellular binding of the TCR to antigen-presenting cells. All CD3 chains, which include CD3G, CD3D, CD3E, and CD3Z, possess immunoreceptor tyrosine-based stimulation motifs in their cytoplasmic structural domains. These motifs undergo phosphorylation when acted upon by the Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition, CD3D functions in thymocyte differentiation. Thymocytes fail to differentiate appropriately without a functioning TCR/CD3 complex. Moreover, previous studies reported that CD3D could act as a biomarker for cancers.

免疫印迹
- WB result of CD3d Rabbit mAb
  Primary antibody: CD3d Rabbit mAb at 1/1000 dilution
  Lane 1: THP-1 whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: HUT 78 whole cell lysate 20 µg
  Negative control: THP-1 whole cell lysate; Raji whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 19kDa
  Observed MW: 21 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD3d antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: Raji
免疫沉淀
- CD3d Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD3d in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD3d Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: CD3d Rabbit mAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
  Predicted MW: 19 kDa
  Observed MW: 21 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD3d antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-CD3d antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
- Negative control: ICC shows negative staining in Raji cells. Anti-CD3d antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).