PDCD6 Recombinant Rabbit mAb (S-578-98)

Programmed cell death protein 6,Apoptosis-linked gene 2 protein homolog,ALG2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0405

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PDCD6
分子别名

Programmed cell death protein 6, Apoptosis-linked gene 2 protein homolog, ALG2
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

O75340
克隆号

S-578-98
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC 1:200
ICFCM 1:50 Hu
ICC 1:500 Hu
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC	1:200
ICFCM	1:50	Hu
ICC	1:500	Hu
IP	1:50	Hu

背景介绍

PDCD6 is a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. It participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death.

免疫印迹
- WB result of PDCD6 Rabbit mAb
  Primary antibody: PDCD6 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: HCT 116 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of PDCD6 Rabbit mAb
  Primary antibody: PDCD6 Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22 kDa
  (This blot was developed with high sensitivity substrate)
- WB result of PDCD6 Rabbit mAb
  Primary antibody: PDCD6 Rabbit mAb at 1/1000 dilution
  Lane 1: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 22 kDa
  Observed MW: 22 kDa
  (This blot was developed with high sensitivity substrate)
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HCT116 (Human colorectal carcinoma epithelial cell) cells labelling PDCD6 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- PDCD6 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PDCD6 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using PDCD6 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: PDCD6 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 22 kDa
  Observed MW: 22 kDa
  （This blot was developed with high sensitivity substrate）
免疫组化
- IHC shows positive staining in paraffin-embedded human breast. Anti-PDCD6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-PDCD6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PDCD6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HCT116 cells. Anti- PDCD6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).